Journal: International Journal of Molecular Sciences
Article Title: Nifedipine Modulates Renal Lipogenesis via the AMPK-SREBP Transcriptional Pathway
doi: 10.3390/ijms20071570
Figure Lengend Snippet: The effect of nifedipine on the cell viability, and intracellular lipid accumulation in NRK52E tubular cells. ( a ) The nifedipine treated cells (15, 30 μM) showed decreased viability compared to control ( p < 0.01) in 24 or 48 h of MTT assays. ( n = 3) ( b ) The nifedipine treated cells (30 μM) and oleic acid (150 μM) showed an increasing effect on quantification of Oil Red O staining comparing to control ( p < 0.01) in 24 or 48 h. ( n = 3) ( c ) The microscopic Oil Red O staining of NRK52E (magnification, 400×) treated with nifedipine and oleic acid are also shown: ( top ) 24 h; and ( bottom ) 48 h. p -values ≤ 0.05 (marked as **) were considered statistically significant. The bar size in ( c ) is 100 μM.
Article Snippet: The normal rat kidney epithelial-derived cell line NRK52E (CRL-1571) was obtained from the Bioresource Collection and Research Center, Food Industry Research Development Institute, Hsinchu, Taiwan.
Techniques: Staining